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	Introduction Gus Rosania Quote Image Start Where Ehrlich Left Supertargeted Styry Library Mitochondria Nucleolus Nucleus Endoplasmic Reticulum Cytoplasm Endolysosomal Compartment Plasma Membrane Listeria Infected Cell Small Changes Take the Magic Away Chemical Adress Tag Are A and B Groups Behaving Binarize the Data Calculate Tag Affinity Sort Affinities Calculate Precision YES! A and B Groups Behave Differential Affinity And Find the Gun What the Gun Looks Like Flourescent Probes Analysis of Subcellular Transport Multidimensional Data Model Systems Dynamics Use Imaging Link Function Parametrize Kinetic Data Assess Fit Across Library A Few Actual Examples Summary Subcellular Localization Where Are We Now Acknowledgements Introduction Cynthia Adams Introduction Image (1) The Cell-Cycle Outline Technology Challenge Hardware Solution Timelapse Acquisition Examples-Control Examples KSP Inhibition Image (2) The Cell Cycle Mitotic Arrest Resulting in Death Mitotic Slippage and Death Cell Fate Phenotypes Mitotic Cell Fate Depends on MOA Fate is a Function of Cell Type Automated Measurements Developed Kinetics of Feature Changes Multivariate Analysis Reveals Patterns Number of Condensed Cells Increases Tracking Cells Between Images (1) Interphase Interphase Duration Normal Division Inhibitor-Induced Increase Decondensation Duration of Mitotic Arrest Tracking Cells Between Images (2) Summary Conclusions Thank You Introduction Anne E. Carpenter New Opportunities Living Cell Microarray Technology Over and Under Expression (1) Over and Under Expression (2) Small Molecule Living Cell Application to Drosophila Genome-Wide Cell-Based Experiments How Can We Identify (1) How Can We Identify (2) Quantitative Automatic Image CellProfiler Modules (1) CellProfiler Modules (2) CellProfiler Modules (3) Improved Identification (1) Improved Identification (2) Cell Edges (1) Cell Edges (2) Application to Images (1) Application to Images (2) Other Applications (1) Other Applications (2) Multiple Parameters Phenotypes of Interest Results (1) Results (2) Future Project Design Thanks To